Didymin Exhibits Different Cytotoxicity Patterns on U-87 MG Human Glioblastoma Cells and Orchestrates the Release of Substance P (SP) and Interleukin-6 (IL-6) in a Time Depending Manner
Naime Bulut1, Ece Simsek21Department of Nutrition and Dietetics, Institute of Health Sciences, Akdeniz University, Antalya, Türkiye, 21Department of Nutrition and Dietetics, Institute of Health Sciences, Akdeniz University, Antalya, Türkiye,
Objectives: This study aims to investigate the cytotoxic effects of didymin on the U-87 MG Human Glioblastoma cell line, besides, its effects on levels of Substance P and Interleukin 6 (IL-6) released from the cells. Methods: The cytotoxic effects of didymin on glioblastoma were determined at the end of 24, 48, and 72 hours incuba tion periods. The effects on the levels of Substance P and Interleukin 6 (IL-6) determined via Elisa. Results: Didymin in the range of 100 – 1,56 ?M/ml doses did not show cytotoxic effects at the end of the 24th and 48th hour. At the end of the 72-hours a cytotoxic effect was observed at a dose of 100 ?M/ml. SP increased at 100 µM/ml and 50 µM/ml doses at the end of the 24 and 48 hours incubation periods and decreased significantly at the end of the 72nd hour. IL-6 increased significantly at 100 ?M/ml at the end of the 24th hour. It caused a significant increase in each dose at the 48th hour, while a significant increase was observed only at the 50 ?M/ml dose at the 72nd hour. Conclusion: The cytotoxic effect of didymin was observed in single dose. Didymin increased the level of inflammation marker IL-6, in a time and dose-dependent manner and decreased the level of SP at the end of the 72nd hour. This was the first study, which exhibits the effects of didymin on SP and IL-6 released from U-87. Keywords: Cytotoxicity, didymin, glioblastoma, substance p, interleukin-6
Cite This Article
Bulut N, Simsek E. Didymin Exhibits Different Cytotoxicity Patterns on U-87 MG Human Glioblastoma Cells and Orchestrates the Release of Substance P (SP) and Interleukin-6 (IL-6) in a Time Depending Manner. EJMO. 2023; 7(4): 326-333
Corresponding Author: Naime Bulut