Immunomodulatory Effects of MDA-MB-231-derived Exosome Mimetic Nanovesicles on CD4+ T Cell Line
Mustafa Oztatlici1, Alper Tunga Ozdemir2, Hulya Oztatlici3, Serife Kucukhuyuk1, Rabia Bilge Ozgul Ozdemir4, Hilmi Orhan5, Kemal Ozbilgin61Department of Histology and Embryology, Faculty of Medicine, Gaziantep Islam Science and Technology University, Gaziantep, Türkiye, 2Department of Medical Biochemistry, Merkez Efendi State Hospital, Manisa, Türkiye, 3Department of Histology and Embryology, Faculty of Medicine, Gaziantep University, Gaziantep, Türkiye, 4Department of Immunology and Allergy, Manisa City Hospital, Manisa, Türkiye, 5Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University, Izmir, Türkiye, 6Department of Histology and Embryology, Faculty of Medicine, Manisa Celal Bayar University, Manisa, Türkiye,
Objectives: The aim of this study is to investigate the immunomodulatory effects of MDA-MB-231 cells or MDA-MB 231-derived exosome-mimetic nanovesicles (NVs) on CD4+ Jurkat cells. Methods: NVs were produced by the breakdown of MDA-MB-231 cells and the characterization of generated NVs were performed by using direct-ELISA and Flow cytometry methods. We co-cultured CD4+ Jurkat cells with MDA-MB-231 cells or MDA-MB-231-derived NVs for 48 h. Subsequently, expressions of pro-inflammatory and anti-inflammatory cy tokines, and related transcription factors of CD4+ Jurkat cells were evaluated by qPCR method. Results: Clustering, which is the indicator of activation, was not seen in the CD4+ Jurkat cells co-cultured with MDA MB-231 cells. However, CD4+ Jurkat cell clusters were observed in the co-culture experiments with all NV concentra tions. In addition, it was determined that the expressions of pro-inflammatory cytokines significantly increased while the expressions of anti-inflammatory cytokines was dramatically decreased in the NV-treated groups. On the contrary, opposite results were obtained in CD4+ Jurkat cells co-cultured with MDA-MB-231 cells. Moreover, TNF-? and Gata3 expressions were decreased in all groups. Conclusion: These preliminary findings from in-vitro experiments suggested that NVs could be a potential tool in can cer immunotherapy, but our data need to be supported by more comprehensive studies. Keywords: Breast cancer, extracellular vesicles, exosome-mimetics, exosomes, immunomodulation
Cite This Article
Oztatlici M, Ozdemir A, Oztatlici H, Kucukhuyuk S, Ozgul Ozdemir R, Orhan H, et al. Immunomodulatory Effects of MDA-MB-231-derived Exosome Mimetic Nanovesicles on CD4+ T Cell Line. EJMO. 2024; 8(1): 40-48
Corresponding Author: Mustafa Oztatlici