Ahead of Print | DOI: 10.14744/ejmo.2023.61011

Therapeutic impact of Nano diosgenin on metabolic reprogramming in an animal model of mammary oncogenesis via modulating carbohydrate metabolizing enzymes

Manobharathi Vengaimaran¹, Kalaiyarasi Dhamodharan¹, Mirunalini Sankaran^1
1Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar,Tamil Nadu, India.,

Objective: The primary target of this study is to explore a novel therapeutic pathway of nano diosgenin by pinpointing the metabolic enzymes that underlies nano diosgenin's anti- breast cancer impacts. Methods: A single dosage of 7,12 Dimethyl Benz(a)anthracene (DMBA) (25 mg/kg b.wt) was injected to induce breast cancer. Oral administration of Diosgenin (DG) (10 mg/kg b.wt) and Diosgenin encapsulated chitosan nanoparticle (DG@CS-NP) (5 mg/kg b.wt) was used to medicate DMBA-induced tumour-bearing rats just after the emergence of a tumour. After the experimental period, biochemical analyses were carried out. Results: Mammary carcinoma-bearing rats showed a significant rise in the levels of glycolytic enzymes (hexokinase, phosphoglucoisomerase, aldolase) and the pentose phosphate pathway enzyme (glucose-6-phosphate dehydrogenase). It also elicits a drop in gluconeogenic enzymes (glucose-6-phosphatase and fructose 1, 6- diphosphatase) and mitochondrial enzymes (succinate dehydrogenase and malate dehydrogenase). Contrarily, nano diosgenin dramatically reverted the rates of glycolytic enzymes, pentose phosphate pathway enzymes, gluconeogenic enzymes, and mitochondrial enzymes in the mammary, liver and kidney tissues to near normal tiers on compared to plain diosgenin treated rats. Thereby, confirming its chemotherapeutic prospects on metabolic rewiring. Conclusion: Thus, our observations suggested that nano diosgenin is a potent therapeutic agent that might have a significant influence on metabolic complications of breast cancer than plain diosgenin. Keywords: Nano diosgenin , Mammary carcinoma , Carbohydrate metabolizing enzymes

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Cite This Article

Vengaimaran M, Dhamodharan K, Sankaran M. Therapeutic impact of Nano diosgenin on metabolic reprogramming in an animal model of mammary oncogenesis via modulating carbohydrate metabolizing enzymes. EJMO. ; (): -

Corresponding Author: Mirunalini Sankaran