Expression Levels of LINC01296 and LINC00152 in Breast Cancer Tissue: Association with the Use of Oral Contraceptives
Mostafa Davoodabadi-Farahani1, Yaser Mansoori2, Shahrzad Ilbeigi3, Maedeh Barahman4, Mohsen Mazloomrezaei5, Pouria Khani6, Seyed MohamMadbagher Tabei1, Seyed Alireza Dastgheib1, Hossein Neamatzadeh71Department of Medical Genetics, Shiraz University of Medical Sciences, Faculty of Medicine, Shiraz, Iran, 2Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran, 3Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, 4Firoozgar Clinical Research Development Center (FCRDC), Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran, 5Cellular and Molecular Students Research Group, Shiraz University of Medical Sciences, Faculty of Medicine, Shiraz, Iran, 6Department of Medical Genetics and Molecular Biology, Iran University of Medical Sciences, Faculty of Medicine, Tehran, Iran, 7Mother and Newborn Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran,
Objectives: Breast cancer is among the most commonly diagnosed cancers and one of the main causes of cancerrelated deaths in females. Long noncoding RNAs (lncRNA) are vital regulators of both oncogenesis and tumor suppression involved in crucial cancer pathways. Recent studies have demonstrated that LINC01296 and LINC00152 were aberrantly upregulated in different tumor types. In this study, we aimed to assess the expression levels of LINC01296 and LINC00152 in breast cancer and its adjacent tissues. Methods: Sample tissues were collected from 49 women with breast cancer referred to Shahid Faghihi Hospital in Shiraz for surgery from 2017 to 2018. Total RNA was extracted from fresh tumor and normal breast cancer tissues and expression of lncRNAs was assessed using real-time polymerase chain reaction. Results: A significant upregulation of LINC01296 and LINC00152 in breast cancer tissues compared with the adjacent normal tissues was observed (p<0.01). The Mann–Whitney analysis showed a significant relationship between the upregulation of LINC01296 and the use of oral contraceptives in luminal B breast cancer subjects (p=0.028). No significant relationship was found between the expression of LINC01296 and quantitative variables. Conclusion: This study showed an upregulation of LINC01296 and LINC00152 in breast cancer tissues compared with the adjacent normal tissues. Keywords: Breast cancer, Long noncoding RNA, LINC00152, LINC01296, Oral contraceptives
Cite This Article
Davoodabadi-Farahani M, Mansoori Y, Ilbeigi S, Barahman M, Mazloomrezaei M, Khani P, et al. Expression Levels of LINC01296 and LINC00152 in Breast Cancer Tissue: Association with the Use of Oral Contraceptives. EJMO. 2022; 6(1): 83-88
Corresponding Author: Seyed Alireza Dastgheib