PIWI Interacting RNA-823: Epigenetic Regulator of The Triple Negative Breast Cancer Cells Proliferation
Cagri Oner1, Ertugrul Colak21Department of Medical Biology and Genetics, Maltepe University, Medical Faculty, İstanbul, Türkiye, 2Department of Biostatistics, Eskişehir Osmangazi University, Medical Faculty, Eskişehir, Turkey Department of Biostatistics, Eskişehir Osmangazi University, Medical Faculty, Eskişehir, Turkey,
Objectives: Triple negative breast cancer cells are estrogen, progesterone, and HER receptor negative, malign breast cancer cells. These cells have high telomerase activity, and this activity gives these cells high proliferation and evading apoptosis abilities. In this study, we aimed to determine the affect piR-823 on genetic parameters of proliferation and ER? status in MDA-MB-231 cells. Methods: After anti-piR-823 transfection, proliferation of cells was tested by XTT. Gene expressions were determined by RT-PCR. Protein expressions were determined by ELISA. Results: The proliferation decreased after inhibition (p<0.001). Gene and protein expressions of ER? were upregulated while hTERT was downregulated after inhibition (p<0.001). Furthermore, piR-823 inhibition cause to decrease PI3K/ AKT/mTOR gene expressions and miR-126 expression (p<0.001). Conclusion: Obtained data indicates that piR-823 inhibition lead MDA-MB-231 cells to increase ER? expression. Decreased expressions of hTERT and PI3K/AKT/mTOR pathway affect cell proliferation. Moreover, miR-126 decrease indicates that piRNAs and miRNAs can share the same target molecules and piRNA expression changes might have an impact on miRNA expressions. All obtained data is important on the perspective of piRNAs and their effect on cellular characteristics of triple negative breast cancer cells. Keywords: Phosphatidylinositol-3-Kinase, piR-823, Triple Negative Breast Cancer
Cite This Article
Oner C, Colak E. PIWI Interacting RNA-823: Epigenetic Regulator of The Triple Negative Breast Cancer Cells Proliferation. EJMO. 2022; 6(4): 339-344
Corresponding Author: Cagri Oner