Association Between Macrophages with Angiogenic Cells and Microvascular Dysfunction in Astrocytic Glioma
Priscilla Das1, Nyi Nyi Naing2, Nadiah Wan-Arfah3, Kon Noorjan4, Kantha Rasalingam51Department of Unit of Biostatistics & Research, Faculty of Medicine, SEGi University, Kota Damansara, Petaling Jaya, Selangor, Malaysia; Department of Methodology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, 2Institute for Community (Health) Development (i-CODE), Universiti Sultan Zainal Abidin, Kuala Nerus, Terengganu, Malaysia, 3Department of Psychiatry, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia, 4Department of Psychiatry, Unit of Biostatistics & Research Methodology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, 5Department of Neuroscience, Hospital Kuala Lumpur, Jalan Pahang, Kuala Lumpur, Malaysia,
Objectives: Astrocytic gliomas are the most common primary brain tumours that developed from glial origin. Macrophages are the predominant inflammatory cells in infiltrating gliomas. The study aimed to investigate the association between circulating macrophages with tissue resident angiogenic cells and microvascular dysfunction in astrocytic glioma. Methods: A total of 22 astrocytic glioma patients were consented from Hospital Universiti Sains Malaysia. Tumour (n=22) were sliced and stained with CD133+ and VEGFA+ angiogenic markers and counter stained with DAPI. The Spearman rho’s test was used for the data analysis. The plasma CD68, macrophage level and von Willebrand factor level or factor VIII level (vWF/ FVIII) was measured using Elisa Kit (CUSABIO BIOTECH CO., LTD). Results: The mean plasma CD68 macrophages was 12.48±19.98 pg/ml. The mean percentage of brain tumour tissue angiogenic cells was (1.26±0.95%), adjacent normal brain tissue angiogenic cells was (0.72±0.68%). Spearman’s rho correlation test showed a significant correlation between brain tumour angiogenic cells and plasma CD68 macrophages (n=22) (Spearman’s rho, r=0.43, p=0.047). However no correlation was observed between plasma CD68 macrophages with adjacent normal brain (Spearman’s rho r =0. 019, p=0. 932). In this study vWF/FVIII level was analysed and about 14 patients with (mild factor level of >5%), 8 patients with (moderate factor level of 1-5%) and no patients had (severe factor level of <1%) was found. The mean vWF/FVIII percentage patient with mild level was 12.48±7.77 % and moderate vWF/FVIII factor level was 3.53±1.32%. The spearman’s rho correlation test showed a significant correlation between patient with moderate vWF/FVIII level with plasma CD68 macrophages (Spearman’s rho r=0.73, p=0.041). Conclusion: Circulating macrophages associated with brain tumour angiogenic cells and vWF/FVIII of astrocytic glioma. Thus targeting these parameters in the treatment of glioma might be useful. Keywords: Angiogenic cells, astrocytic glioma, brain tumour, macrophages, microvascular dysfunction
Cite This Article
Das P, Naing N, Wan-Arfah N, Noorjan K, Rasalingam K. Association Between Macrophages with Angiogenic Cells and Microvascular Dysfunction in Astrocytic Glioma. EJMO. 2021; 5(1): 37-42
Corresponding Author: Priscilla Das