Genetic Markers Indicate that 1,25-dihydroxyvitamin D Treatment may not Protect Against Hepatocellular Carcinoma
Cagri Oner1, Ertugrul Colak21Department of Medical Biology and Genetics, Maltepe University Faculty of Medicine, Istanbul, Turkey Department of Medical Biology and Genetics, Maltepe University Faculty of Medicine, Istanbul, Turkey, 2Department of Biostatistics, Eskisehir Osmangazi University Faculty of Medicine, Eskisehir, Turkey,
Objectives: The impact of 1,25-dihydroxyvitamin D on hepatocellular carcinoma (HCC) cells is a complicated area. In this study, we aimed to clarify the effect of 1,25-dihydroxyvitamin D on HCC cells according to genetic markers. Methods: The optimal concentration of 1,25-dihydroxyvitamin D is treated to HepG2 cells (250 nM at the 48th hour). From treated HepG2 cells, total Ribonucleic Acid (RNA) was isolated, and Ki-67, MMP-2, MMP-9, HIF-1?, hTERT, and piR823 gene expressions were determined by SYBR Green-based real-time polymerase chain reaction. Results: : Increased expressions of Ki-67, hTERT, and piR-823 were determined compared with the control group at the 48th hour after treatment (p<0.001), while decreased gene expressions of MMP-2, MMP-9, and HIF-1? were observed compared with the control group (p<0.001). Conclusion: Currently, there are several different opinions about the usage of vitamin D, especially in HCC. In addition to researchers who argue that vitamin D has anticarcinogenic and protective properties, an increasing number of researchers argue that tumor cells can become aggressive after HCC occurs. According to our results, it was determined that vitamin D causes HepG2 HCC cells to become aggressive in terms of gene expression in the parameters used as a marker for proliferation, adhesion, and differentiation. Keywords: 1.25-Dihydroxyvitamin D, hepatocellular carcinoma, motility, PIWI interacting RNA, proliferation
Cite This Article
Oner C, Colak E. Genetic Markers Indicate that 1,25-dihydroxyvitamin D Treatment may not Protect Against Hepatocellular Carcinoma. EJMO. 2021; 5(2): 117-122
Corresponding Author: Cagri Oner