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Modulation of Vascular Endothelial Growth Factor and Tissue Necrosis Factor Alpha Using Ethanol Stem Bark Extract of Boswellia Dalzielii H. Attenuates Ethanol-induced Gastric Ulcer in Albino Rats [EJMO]
EJMO. 2018; 2(2): 97-104 | DOI: 10.14744/ejmo.2018.74745

Modulation of Vascular Endothelial Growth Factor and Tissue Necrosis Factor Alpha Using Ethanol Stem Bark Extract of Boswellia Dalzielii H. Attenuates Ethanol-induced Gastric Ulcer in Albino Rats

Anas Husainy Yusuf1, Hamza A Salami1, Maryam Babakura2, Umar Kyari Sandabe3, Nuhu Sambo4, Abdulwasiu Abubakar1
1Department of Human Physiology, College of Medical Sciences, University of Maiduguri, Nigeria
2Department of Biological Science, Faculty of Science, University of Maiduguri, Nigeria
3Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine,
4Department of Human Physiology, Faculty of Medical Sciences,Bingham University, Nigeria

Objectives: The modulation of vascular endothelial growth factor, antioxidants, and tissue necrosis factor alpha (TNF-α) using ethanol stem bark extract of Boswellia dalzielii H. was evaluated in an ethanol-induced gastric ulcer albino rat model.
Methods: Thirty albino rats of either sex (200250 g) were starved for 48 h but were allowed drinking water with 8% sucrose to avoid dehydration. The rats were placed on wire gauze above the base of the cage to prevent coprophagy. At the end of the fasting period, the rats were equally divided and assigned to six treatment groups. Group A served as control and 5 ml/kg distilled water was orally administered to the rats without further treatment. Rats in group B were given 5 ml/kg distilled water and served as negative control. Rats in groups C, D, and E were pretreated with 100, 200, and 400 mg/kg of the ethanol stem bark extract of B. dalzielii H, respectively. Group F received 50 mg/kg ranitidine. After 1 h, all the rats in groups BF were each given absolute ethanol 1 ml/200 g body weight of rat. All treatments were by intragastric lavage. One hour after the treatment with ethanol, all the rats in the experiment (groups AF) were euthanized with an overdose of anesthetic ether and their stomachs were excised. The stomachs were cut along the greater curvature and washed in warm normal saline. Each stomach was stretched out and pinned on board.
Results: The results of the study revealed that pretreatment with ethanol stem bark extract of B. dalzielii H. decreased gross and histological gastric mucosal damage caused by intragastric administration of absolute ethanol in a dose-dependent manner when compared with controls. The gastric ulcer index and gastric tissue level of malondialdehyde (MDA) and TNF-α were significantly reduced (p<0.001), whereas the gastric tissue level of superoxide dismutase, catalase, total antioxidant capacity, and vascular endothelial growth factor (VEGF) was significantly increased (p<0.001) when compared with the controls.
Conclusion: The plant extract attenuated gastric mucosal damage induced by ethanol via upregulation in the expression of gastric tissue VEGF, reinforcement of the antioxidant system, and reduction in the gastric tissue level of MDA and the pro-inflammatory cytokine TNF-α.

Keywords: Antioxidants, ethanol, gastric ulcer, tissue necrosis factor alpha, vascular endothelial growth factor

Anas Husainy Yusuf, Hamza A Salami, Maryam Babakura, Umar Kyari Sandabe, Nuhu Sambo, Abdulwasiu Abubakar. Modulation of Vascular Endothelial Growth Factor and Tissue Necrosis Factor Alpha Using Ethanol Stem Bark Extract of Boswellia Dalzielii H. Attenuates Ethanol-induced Gastric Ulcer in Albino Rats. EJMO. 2018; 2(2): 97-104

Corresponding Author: Anas Husainy Yusuf, Nigeria
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